Faculty of Pharmacy and Pharmaceutical Sciences

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This unit entry is for students who completed this unit in 2016 only. For students planning to study the unit, please refer to the unit indexes in the the current edition of the Handbook. If you have any queries contact the managing faculty for your course or area of study.

Monash University

6 points, SCA Band 2, 0.125 EFTSL

Undergraduate - Unit

Refer to the specific census and withdrawal dates for the semester(s) in which this unit is offered.


Pharmacy and Pharmaceutical Sciences


Dr Laurence Orlando



  • First semester 2016 (Day)


The objective of this unit is to provide students with opportunities to develop their analytical skills by testing various hypothesis and strategies to solve authentic complex HPLC problems.

On successful completion of this unit, students will have developed a systematic and critical approach to analytical method development that will be demonstrated by the creation of relevant and accurate resources usable in future jobs or studies.


At the end of this unit students will be able to:

  1. Explain in details each HPLC module function and the functioning of the integrated system to confidently operate the HPLC instrument (hardware software) to obtain accurate chromatograms
  2. Raise hypotheses on the impact of controllable factors on HPLC responses, design and conduct experiments to test these hypotheses
  3. Critically evaluate, analyse and interpret the collected results to generalise the rules that govern HPLC
  4. Explain in details the concepts linked to resolution, retention factor, selectivity, efficiency, peak tailing and other secondary responses and generalise the rules that govern HPLC
  5. Create a concept map showing the complexity of the interactions between controllable factors and essential responses
  6. Create a flow chart showing the complex decision making process necessary to develop a HPLC method
  7. Explain and apply the principles of analytical method validation


In-semester assessment 100%

Workload requirements

Contact hours for on-campus students:

  • twenty-four hours of practicals
  • Forty-eight hours of workshops

See also Unit timetable information

Chief examiner(s)

Additional information on this unit is available from the faculty at: