Postgraduate - UnitMCE5112 - IVF processes: embryo production and cryopreservation techniques (practical)

This unit entry is for students who completed this unit in 2013 only. For students planning to study the unit, please refer to the unit indexes in the the current edition of the Handbook. If you have any queries contact the managing faculty for your course or area of study.

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12 points, SCA Band 2, 0.250 EFTSL

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Level	Postgraduate
Faculty	Faculty of Medicine, Nursing and Health Sciences
Organisational Unit	Department of Obstetrics and Gynaecology
Offered	Clayton First semester (extended) 2013 (Day) Clayton First semester (extended) 2013 (Off-campus)
Coordinator(s)	Dr Mulyoto Pangestu

Synopsis

The theoretical aspects of IVF, embryo production and cryopreservation will be covered in the previous unit reinforced by extensive practical training The theoretical knowledge gained in slow cooling and vitrification techniques will also enhanced by practical training. Students will freeze, thaw and analyse sperm samples, oocytes and embryos Students will gain a better understanding of media components by making and designing their own media. This unit enables students to develop and enhance their embryo handling skills.

Outcomes

On completion of this unit students will:

Have a broad theoretical knowledge about the components of culture systems competently demonstrate skills in preparing media suites for in vitro culture of embryos
Show competence in handling mouse oocytes and embryos and be able to perform mouse IVF using epididymal sperm
Develop practical skills in the techniques of oocyte maturation, sperm preparation by gradient and swim up, and insemination and IVF using frozen thawed ejaculated sperm using ovine gametes Be able to demonstrate practical competence in the techniques of vitrification and slow cooling of embryos and gametes and to plan experiments to compare different freezing methods
Have the skills to critically analyse research papers in cryopreservation related to human reproduction

Assessment

Practical skills on IVF (25%) and cryopreservation (25%)
Lab book assessment (10%)
Quiz (20%)
Research plan for project (20%)

Chief examiner(s)

Dr Mulyoto Pangestu

Contact hours

30h contact per week (research,tutorials, self-directed study, practical skill training.