MCE1200

Practical techniques and statistics in embryology II ( 12 points, SCA Band 2, 0.250 EFTSL)

Leader: Dr Peter Sjoblom

Offered:
Clayton 2-30 2005 (Day)
Clayton 2-32 2005 (Day)

Synopsis: Students gain skills and experience in the practical techniques of micromanipulation, molecular biology and the analysis of this data. Techniques include preparation of micromanipulation instruments, the handling of sperm after its extraction from ejaculates or testicular biopsies prior to its use in IVF, the performance of ICSI, and the identification of Y-chromosome deletions in the sperm. Also taught will be the importance of molecular biology to the study of genomic imprinting, and to the performance of transgenesis and parthenogenesis, as alternative methods of reproduction, and to the detection of genetic and developmental abnormalities, using FISH, PCR and stage specific-markers.

Objectives: On completion of this unit students will: 1. be familiar with the applications of the practical techniques to be learnt and the statistical methods used for analysis of the data generated by the application of these techniques; 2. be familiar with the applications of micromanipulation instruments; 3. understand the applications of FISH and karyotyping; 4. have a practical knowledge of the techniques used for the treatment of male infertility and ICSI; 5. be aware of the methods used for the preparation of sperm from ejaculates and testicular biopsy samples; 6. demonstrate the extraction of DNA from cells and the skills required for carrying out PCR and genetic analysis; 7. be competent in the handling and maintenance of in vitro embryonic stem cell lines; 8. demonstrate the ability to carry out transgenesis and identification of transgenic clones; 9. understand the methods used for pathenogenesis.

Assessment: Demonstration of practical competence in techniques: 100%

Contact Hours: 18 weeks (1.5 days laboratory session each week, 6 hours videos, discussions, technical demonstrations over the semester)