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MCE1200

Practical techniques and statistics in embryology II (12 points)

(MED)

Leader: Dr Peter Sjoblom

Offered:
Clayton 2-30 2004 (Day)
Clayton 2-30 2005 (Day)

Synopsis:

Objectives: On completion of this unit students will: 1. be familiar with the applications of the practical techniques to be learnt and the statistical methods used for analysis of the data generated by the application of these techniques; 2. be familiar with the applications of micromanipulation instruments; 3. understand the applications of FISH and karyotyping; 4. have a practical knowledge of the techniques used for the treatment of male infertility and ICSI; 5. be aware of the methods used for the preparation of sperm from ejaculates and testicular biopsy samples; 6. demonstrate the extraction of DNA from cells and the skills required for carrying out PCR and genetic analysis; 7. be competent in the handling and maintenance of in vitro embryonic stem cell lines; 8. demonstrate the ability to carry out transgenesis and identification of transgenic clones; 9. understand the methods used for pathenogenesis.

Assessment: Demonstration of practical competence in techniques: 100%

Contact Hours: 18 weeks (1.5 days laboratory session each week, 6 hours videos, discussions, technical demonstrations over the semester)


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