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MCE1112

Embryo culture and cryopreservation (6 points)

(MED)

Leader: Dr Peter Sjoblom & Dr Jill Shaw

Offered:
Clayton 1-30 2004 (Day)
Clayton 1-30 2005 (Day)

Synopsis:

Objectives: On completion of this unit students will: 1. appreciate the importance of asepsis and quality control when setting up an IVF laboratory; 2. be familiar with the guidelines recommended for setting up a quality IVF laboratory that produces increased pregnancy rates; 3. have a knowledge of the various quality control tests that are used routinely in an IVF laboratory, such as mouse bioassays, sperm survival tests, etc.; 4. possess a sound theoretical knowledge about the preparation of simple and complex media for in vitro culture and co-culture systems; 5. have gained an understanding about the scoring and selection of good from bad quality embryos from the 2-cell stage of embryogenesis through to hatching blastocysts, based on morphological and metabolic characteristics; 6. possess a sound theoretical knowledge and understanding of the techniques of gamete and embryo cryopreservation as used in a clinical human in vitro fertilisation program; 7. have the skills to critically analyse research papers in cryopreservation related to human reproduction; 8. have the ability to communicate this knowledge.

Assessment: Written examination (2 hours): 50% + Presentation of journal review: 20% + Essay(2500 words): 30%

Contact Hours: 6 weeks (first 5 weeks - two 1-hour lectures, 1-hour tutorial, 1.5-hour seminar, 1.5-hour journal review), 2-hour revision tutorial in week 6


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