BTH3757

Molecular biology and biotechnology

(SCI)

Dr Philip Brook-Carter

6 points + Second semester + 3 hours of lectures per week and 40 hours laboratory work per semester + Gippsland, OCDL + Prerequisites: BTH2746 or BCH2011 or BCH2031 + Prohibitions: BCH3011, BCH3031, ASC3657

Synopsis: This subject concentrates on important areas of the methodology of biochemical investigation and recombinant DNA methodology, with a strong emphasis on techniques of recombinant DNA manipulation which ensures students achieve working familiarity with a range of procedures including gene cloning, nucleic acid hybridisation (in vitro and in situ), PCR technology and DNA fingerprinting. The applications of these techniques to a range of industrial and research activities are considered. These include the genetic manipulation of plants and animals, gene therapy, detection and typing of viruses, production of recombinant vaccines, environmental monitoring, and industrial biocatalysis.

Assessment: Final examination (3 hours): 50% + Assignments: 20% + Practical work: 30% + A pass in both the theory and practical components is mandatory