Applied biochemistry
Ms Jenny Mosse
6 points * Second semester * Four 1 hour lectures and one 45-hour laboratory block, performed over 5 consecutive days * Gippsland/Distance * Prerequisites: GAS2141
Objectives On the completion of this subject students will be able to understand the molecular basis of a wide range of recombinant DNA techniques; demonstrate proficiency in these techniques; appreciate the use of a range of biochemical, spectroscopic and molecular biological techniques in the diagnosis of disease; demonstrate proficiency in the interpretation of complex data acquired by these methods; apply biochemical knowledge acquired in prior study to the pursuit of current scientific problems in industry, medicine and research; critically evaluate a variety of approaches to a particular (biochemical) problem.
Synopsis This subject concentrates on important areas of the methodology of biochemical investigation and recombinant DNA technology. This includes methods of clinical diagnosis such as NMR, immunoassay and automated analysis. There is a strong emphasis on techniques of recombinant DNA manipulation, which ensures students achieve working familiarity with a range of procedures. This practical program is supported by an extensive theory program which includes several detailed case studies.
Assessment Mid-semester test and final examination: 70% * Practical work: 30% * A pass in both theory and practical work is mandatory.
Prescribed texts
Brown T A Gene cloning: An introduction 3rd edn, Chapman and Hall, 1995
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