Faculty of Pharmacy and Pharmaceutical Sciences

Monash University

Undergraduate - Unit

This unit entry is for students who completed this unit in 2015 only. For students planning to study the unit, please refer to the unit indexes in the the current edition of the Handbook. If you have any queries contact the managing faculty for your course or area of study.

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6 points, SCA Band 2, 0.125 EFTSL

Refer to the specific census and withdrawal dates for the semester(s) in which this unit is offered.

FacultyFaculty of Pharmacy and Pharmaceutical Sciences
OfferedParkville First semester 2015 (Day)
Coordinator(s)Dr Laurence Orlando


The objective of this unit is to provide students with an opportunity to develop their analytical skills by testing various hypothesis and HPLC strategies to solve an authentic complex HPLC problem.

On successful completion of this unit, students will have developed a systematic and critical approach to analytical method development and validation that will be demonstrated by the creation of relevant and accurate resources usable in future jobs or studies.


At the end of this unit students will be able to:

  1. Precisely operate standard laboratory equipment to conduct accurate volumetric dilutions and filtration to prepare samples and standard solutions
  2. Explain in details each HPLC module function and the functioning of the integrated system to confidently operate the HPLC instrument (hardware software) to obtain accurate chromatograms
  3. Explain in details the concepts of resolution, retention factor, selectivity, efficiency, peak tailing and other secondary responses and predict the impact of controllable factors on each of these responses
  4. Design and conduct experiments to test these hypotheses,
  5. Critically analyse and interpret the collected results to generalise the rules that govern HPLC
  6. Create a concept map showing the complexity of the interactions between controllable factors and essential responses
  7. Create a flow chart showing the complex decision making process necessary to develop a HPLC method in the context of reverse phase chromatography
  8. Explain the principles of method validation and apply principles to a set of data to write a method validation report following ICH guidelines.

(11)Report on analysis, evaluation, interpretation and synthesis of validation results and draw conclusions on validation acceptance criteria.

(12)Develop a systematic approach for troubleshooting HPLC systems.


In-semester assessment 100%

Workload requirements

Contact hours for on-campus students:

  • Thirty two hours of practicals
  • Forty hours of workshops

See also Unit timetable information

Chief examiner(s)


PSC2031 Analytical methods

Additional information on this unit is available from the faculty at: