Undergraduate - UnitPSC3131 - Spectroscopy

After completing this unit students will be expected to be able to:

1. Analyse and interpret two dimensional spectra so as to identify the chemical structures of compounds;
2. Define the term Nuclear Overhauser Enhancement (NOE) and account for the observation of NOEs in one-dimensional and two-dimensional NMR spectra of both small and large molecules;
3. Analyse and interpret NOE data to determine the conformation of small molecules;
4. Analyse and assign two-dimensional NMR spectra of small peptides;
5. Describe quantitatively the relationship between enthalpy, entropy and free energy;
6. Describe quantitatively the relationship between changes in free energy and equilibrium;
7. Apply the concepts of this thermodynamics module to selected examples of biochemical energetics, protein-drug binding and drug-receptor interactions;
8. Describe the principal NMR-based strategies for drug discovery and design;
9. Detail the factors which govern photon-initiated electronic excitation, and describe the processes by which molecules can relax. In particular, to describe the phenomena and applications associated with fluorescence;
10. Apply their knowledge of biophysical techniques including NMR spectroscopy, fluorescence spectroscopy, surface plasmon resonance and calorimetry to analyse experimental data describing drugs binding to their receptors
11. Measure and record data relevant to the understanding of drug structure and and drug-receptor interactions;
12. Perform numerical calculations based on experimental or theoretical data;
13. Present written or oral results of experimental work.