Undergraduate - UnitPSC3041 - Applied analytical methods

At the end of this unit students will be able to:

1. Precisely operate standard laboratory equipment to conduct accurate volumetric dilutions and filtration
2. Appropriately label equipment, design and codify GLP documentation
3. Effectively operate an HPLC instrument
4. Develop teamwork skills including group contracting, goal setting and project planning, decision making and conflict management
5. Prepare a clear plan of action to solve a given problem and justify the experimental design
6. Accurately perform the experiments, compile the results and critically analyse, interpret and theorize on the observed phenomena
7. Select correct capacity factor (k') and efficiency (N) to improve resolution and minimise band broadening;
8. Compare and select the most effective solvent, columns and stationary phase chemistry, to improve selectivity and subsequently separation resolution to finalise an efficient HPLC method
9. Compare and contrast isocratic and gradient methods and develop a gradient method to optimise the run time
10. Describe the principles of method validation and prepare a method validation protocol taking into account the ICH guidelines;
11. Report on analysis, evaluation, interpretation and synthesis of validation results and draw conclusions on validation acceptance criteria.
12. Develop a systematic approach for troubleshooting HPLC systems.