Faculty of Science

Monash University

Undergraduate - Unit

This unit entry is for students who completed this unit in 2014 only. For students planning to study the unit, please refer to the unit indexes in the the current edition of the Handbook. If you have any queries contact the managing faculty for your course or area of study.

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6 points, SCA Band 3, 0.125 EFTSL

Refer to the specific census and withdrawal dates for the semester(s) in which this unit is offered, or view unit timetables.

FacultyFaculty of Science
OfferedClayton First semester 2014 (Day)
Coordinator(s)Dr Kim Murphy


This unit aims to introduce students to practical aspects of laboratory immunology. The course is laboratory based with tutorials to ensure a thorough theoretical understanding of techniques. Topics include the anatomy of the immune system, production of polyclonal antisera and monoclonal antibodies, structure and properties of immunoglobulins (antibodies), electrophoresis and other techniques for detection of antigen/antibody reactions. Diagnostic immunology techniques include immunofluorescence, fluorescence microscopy, flow cytometry, enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and analysis of plasma proteins (immunoglobulins, complement etc).


On completion of this unit students will be able to:

  1. Compare different methods of antibodies purified from a range of different biological sources;

  1. Validate different methods of testing purified antibody products;

  1. Demonstrate the ability to perform common laboratory calculations and use these in a practical setting;

  1. Accurately record, analyse and report experimental outcomes and demonstrate this by producing written laboratory reports;

  1. Source relevant scientific literature from a variety of sources, including (but not limited to) scientific journals, text-books and the internet for the completion of written or oral assignments.


Practical class reports: 25%
One assignment: 15%
One 3-hour practical/written examination: 60%

Chief examiner(s)

Workload requirements

One 5-hour practical class per week

This unit applies to the following area(s) of study


IMM2011 or MIC2022 or BMS2052 or BND2042


IMM3031 (highly recommended)