MCE1212 - Preimplantation genetic diagnosis and embryo gene expression

Leader: Dr Sally Catt

Offered

Clayton Second semester (extended) 2007 (Day)

Synopsis

Provides information about the molecular biology and genetic techniques essential in a clinical human IVF program. Students will be taught the theoretical basis of current techniques in genetic diagnosis, polymerase chain reaction (PCR) and fluorescent in situ hybridisation (FISH), and the applications of embryonic stem cell lines. On completion, students will possess a sound theoretical knowledge and understanding of the molecular and genetic techniques used in a clinical human genetics laboratory, and also become familiar with the different applications of these techniques.

Objectives

On completion of this unit students will:

1. possess a sound theoretical knowledge and understanding of the techniques essential to chromosomal and molecular genetic analysis in a clinical human IVF program;
2. have the skills to critically and constructively analyse research papers in molecular biology and reproduction;
3. understand the applications of polymerase chain reaction (PCR) and fluorescent in situ hybridisation (FISH);
4. show an understanding of the applications of embryonic stem cell lines in research;
5. be familiar with the methods of genetic analysis applicable in a clinical fertilisation program;
6. have the ability to communicate this knowledge.

Assessment

Written examination (1.5 hours): 50%
Presentation of journal review: 20%
Essay (2,500 words): 30%

Contact hours

6 weeks (first 5 weeks - two 1-hour lectures, 1-hour tutorial, 1.5-hour seminar, 1.5-hour journal review), 2-hour revision tutorial in week 6