BTH3757 - Molecular biology and biotechnology

Leader: Dr Phillip Brook-Carter

Offered

Gippsland Second semester 2007 (Day)
Gippsland Second semester 2007 (Off-campus)
Malaysia First semester 2007 (Day)
Malaysia Second semester 2007 (Day)

Synopsis

This unit concentrates on important areas of the methodology of biochemical investigation and recombinant DNA methodology, with a strong emphasis on techniques of recombinant DNA manipulation which ensures students achieve working familiarity with a range of procedures including gene cloning, nucleic acid hybridisation (in vitro and in situ), PCR technology and DNA fingerprinting. The applications of these techniques to a range of industrial and research activities are considered. These include the genetic manipulation of plants and animals, gene therapy, detection and typing of viruses, production of recombinant vaccines, environmental monitoring, and industrial biocatalysis.

Objectives

On completion of this unit students will understand endocrine control of cellular processes; appreciate the biochemical perturbations in some important disease states; appreciate the use of biochemical and spectroscopic techniques in the diagnosis of disease; be able to plan and execute complex biochemical laboratory procedures; demonstrate proficiency in the interpretation of complex data acquired by biochemical and spectroscopic techniques; demonstrate basic competence in the safe handling of hazardous biological materials and radioisotopes.

Assessment

Final examination (3 hours): 50%
Assignments: 20%
Practical work: 30%
A pass in both the theory and practical components is mandatory

Contact hours

3 hours of lectures per week and 40 hours laboratory work per semester

Prerequisites

BTH2746 or BCH2011 or BCH2031

Prohibitions

BCH3011, BCH3031, ASC3657