ANT3032 - Medical laboratory technology

Leader: Dr M Jane Black

Offered

Clayton Second semester 2007 (Day)

Synopsis

ANT3032 covers a number of fundamental techniques routinely used in biomedical research, in particular, techniques for studying cell biology. This subject provides the background knowledge required for the understanding and implementation of widely used medical laboratory techniques. The practical classes involve basic experiments utilising these techniques. An important aspect of the subject is the analysis of experimental data and the preparation of scientific reports.

Objectives

On completion of this unit, students will have a sound practical and theoretical knowledge of aspects of contemporary biomedical techniques. Specifically they will have an understanding of: fundamental histological techniques including fixation, processing, embedding, sectioning and staining. Light microscopy including fluorescence and confocal microscopy. Electron microscopy. Tissue/cell and organ culture; including isolation of cells from tissues, primary culture and maintenance of cells/tissues in culture. Contemporary approaches to the investigation of cell growth, proliferation and apoptosis. Methods for localising specific molecules and genes in tissues and sections including immunohistochemistry and in situ hybridisation. Methods for quantifying gene and protein expression in tissues including real time PCR and Western bloc analysis. The interrelationship between biochemical and cellular techniques, quantitation of histological parameters. Principles of experimental design. Preparation of a scientific report.

Assessment

Examination (2 hours MCQ): 50%
Written reports of weekly practical classes: 30%
Written assignment (1500 words): 20%

Contact hours

Three 1-hour lectures per week and One 3-hour practical class per week

Prerequisites

24 points of level two units (or equivalent) or with permission

Prohibitions

ANT3011